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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Ann Biomed Eng. 2013 Aug 27;42(1):149–161. doi: 10.1007/s10439-013-0894-3

Figure 2.

Figure 2

Characterization of membrane culture system and EndMT response to TNFα. A. Western blot analysis of a random sampling of four of the populations of PAVEC used in the study. Populations of PAVEC were sampled for western blot analysis of protein content at passage three and used in experiments at passages four to six. All populations showed strong VE-cadherin expression and no αSMA expression. B, C. 3µm and 8µm membrane pores, scale bar is 20µm. D. PAVEC cultured on the membrane system for six days retain strong CD31 expression, no αSMA expression, and cobblestone morphology. Scale bar is 20µm. E, F. PAVEC cultured on the membrane system in control conditions for six days do not express αSMA and do not pass through the membrane into the hydrogel. G. After 48 hours of TNFα treatment, a subset of PAVEC invade through the membrane into the hydrogel (arrows) and a subset remains on the membrane.