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. 2014 Jan 29;8:3. doi: 10.3389/fncel.2014.00003

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Copyright © 2014 Rokic, Stojilkovic and Zemkova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protocol 2: the effect of cadmium on sustained current response by mutant rP2X4Rs. The whole-cell current recordings from -60 mV represent the effects of 20 μM cadmium transiently co-applied (gray field) with 100 μM ATP. Cadmium application was initiated at 50 ± 10% of current desensitization decay. (A) Enhancement of cadmium effects in cysteine mutants (red traces) and the corresponding alanine mutants (blue traces). (B) A transient inhibition of sustained current by cadmium (red traces). Right panels show the response of the corresponding alanine mutants. Vertical dotted lines illustrate the duration of ATP application in cells expressing the WT receptor. Traces shown are representative and mean ± SEM values for these mutants are shown in Table 1.