Table 1.
Summary and characteristic of all magnetosome-associated proteins that are encoded in Magnetospirillum gryphiswaldense MSR-1strain.
| Protein | Number of amino acid | Transmembrane helices | Domain | Predicted structure template | % Structure confidence or sequence identity (E value) | Major function in |
|---|---|---|---|---|---|---|
| MamE | 591 | 1 (27–48) | Magnetochrome, trypsin and PDZ | 2Z9I | 100% | Biomineralization |
| MamT | 174 | 1 (10–28) | Magnetochrome | Secondary structure only | – | Biomineralization |
| MamO | 632 | 8 (1-N-terminal, 7-C-terminal) | Trypsin and anion transport | 1LCY | 17% | Biomineralization, iron transport |
| MamH | 428 | 12 | MFS, similar to MamZ | 2XUT, 4JOD (PiPT) | 100% | Biomineralization |
| MamI | 77 | 2 | No conserved domain | Secondary structure only | – | Membrane invagination |
| MamP | 270 | 1 (N-terminal) | PDZ, magnetochrome | 4JJ3 (MamP, MO-1) | Structure was determent | Biomineralization |
| MamN | 437 | 11 | Na+/H+ antiporter | 4F35 (monomer) | 100% | Biomineralization, iron transport and nucleation |
| MamD | 314 | 1 (C-terminal) | No conserved domain | 3C12 (FlgD) – only N-terminal | 77.10% | Crystal shape |
| MamM | 318 | 3 | CDF, metal carrier | 3H90 (FieF) | 100% | Iron transport |
| MamB | 297 | 3 | CDF, metal carrier | 3H90 (FieF) | 100% | Iron transport |
| MamQ | 273 | 1 (N-terminal) | LemA protein | 2ETD (LemA) | 100% | MM formation |
| MamR | 72 | – | DNA-binding | 2KFS (RV2175c) | 95.50% | Crystal shape |
| MamS | 180 | 1 (N-terminal) | OB fold | 1NNX (YgiW) | 91.80% | Post-nucleation |
| MamZ | 661 | 18 | YedZ- like domain (C-terminal), MFS (N-TER) | 1PW4 (GlpT), 3CX5 (cytochrome C) | N-terminal (100%), C-terminal (32.86%) | Biomineralization (C-terminal) |
| MamJ | 426 | – | CAR domain mostly unstructured, TonB | Unstructured protein | – | Magnetosome arragment into chain structure |
| MamK | 360 | Filaments structure (MreB, ParM)-actin-like, ATP binding site | 1JCF (MreB) | 100% | Magnetosome arragment into chain structure | |
| MamL | 123 | 2 | Similar to MamI | Secondary structure only | – | Membrane invagination |
| MamA | 217 | – | TPR | 3AS4 (MamA, AMB-1), 3AS8 (MamA, MSR-1), 2MUC | Structure was determent | Surrounded the magnetosome vesicles |
| (MamA, M. bavaricum) | ||||||
| MamU | 297 | – | DGKs | 3T5P | 2.00E-15 | Membrane invagination |
| MamX | 269 | 1 (N-terminal) | Similar to MamE and MamS, cytochrome, DNA-binding domain | 1OGY, 1JNI, 3ML1, 1QO8 (cytochrome C) 2CQA, 3KDF, 3KF6 (OB fold) | 90% (N-terminal) | Crystal maturation |
| 69% (C-terminal) | ||||||
| MamY | 371 | 2 (N-terminal) | BAR domain | 1SJ8 (talin) | 95.40% | Membrane invagination |
| FtsZ like | 323 | – | GTPase actin domain | 2RHL | 1.00E-92 | Crystal size and cell division |
| MamC | 125 | 2 | No conserved domain | Secondary structure only | – | Crystal size and shape |
| MamG | 84 | 2 | Similar to MamD and Mms6 | Secondary structure only | – | Crystal size and shape |
| MamF | 124 | 3 | Similar to MmsF | Secondary structure only | – | Crystal size and shape |
| Mms6 | 136 | 1 | C-terminal highly acidic involved in nucleation | Base on energy calculations of the sequence | Crystal size and shape |