Table 1. Summary of mRNA levels and DNA binding of TET1 (a) and DNMT1 (b) to the RELN and GAD1 promoters in cerebella of ASD vs CON.
| (a) |
ChIP (% of DNA input) |
(b) |
ChIP (% of DNA input) |
|||
|---|---|---|---|---|---|---|
| TET1 relative mRNA | TET1 binding to RELN | TET1 binding to GAD1 | DNMT1 relative mRNA | DNMT1 binding to RELN | DNMT1 binding to GAD1 | |
| CON | 0.021±0.010 | 0.011±0.021 | 0.070±0.011 | 0.76±0.25 | 0.20±0.010 | 0.11±0.020 |
| ASD | 0.052 ±0.010 | 0.031±0.064 | 0.100±0.013 | 0.60±0.11 | 0.16±0.030 | 0.13±0.020 |
| P-value | 0.01 | 0.01 | 0.046 | 0.6 | 0.5 | 0.6 |
Abbreviations: ASD, autism spectrum disorder; ChIP, Chromatin ImmunoPrecipitation; CON, control. % of DNA input was calculated as 2(Ct(10% input)−3.32)−Ct(gene−IP) × 100% where IP is the amount of specific gene-immunoprecipitated DNA and input is the amount of the same gene in the starting material. Relative mRNA levels were calculated relative to the amounts of GAPDH mRNA.