Table 2.
Fold change in expression level of selected genes in Pax3 Spd / Spd mutant humeri compared to control humeri revealed by Microarray, RNA seq and qRT-PCR
| Gene symbol | Gene name | Regulation status | Microarray (Fold change) | RNA-seq (Fold change) | qRT-PCR (Fold change) |
|---|---|---|---|---|---|
|
Fgf4 |
Fibroblast growth factor 4 |
Down-regulated |
41.05 |
#No numerical value |
35.24*** |
|
Cilp |
Cartilage intermediate layer protein, nucleotide pyrophosphohydrolase |
Down-regulated |
9.73 |
9.76 |
11.3*** |
|
Rxrg |
Retinoid X receptor gamma |
Down-regulated |
7.05 |
12.29 |
5.25** |
|
Dll1 |
Delta-like 1 |
Down-regulated |
2.21 |
3.16 |
1.78* |
|
Spp1 |
Secreted phospoprotein 1 |
Down-regulated |
5.23(ns) |
2.9 |
2.59* |
|
Vstm2a |
V-set and transmembrane domain containing 2A |
Up-regulated |
8.56 |
3.09 |
14.17** |
|
Figf |
c-fos induced growth factor |
Up-regulated |
3.52 |
3.17 |
4.06** |
|
Fgf10 |
Fibroblast growth factor 10 |
Up-regulated |
2.48 |
2.14 |
3.28 |
| Sfrp2 | Secreted frizzled-related protein 2 | Up-regulated | 2.62 | 2.09 | 3.38* |
#No numerical value for fold change as no transcripts detected in the mutant.
***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 indicates significance of differential expression from qRT-PCR.
p ≤ 0.05 for all microarray and RNA-seq differences except Spp1 in the Microarray (ns).