Table 2.
RIN of tissue sections or PTCs at progressive stages of sample preparation
| Experimental conditions | RIN |
|---|---|
| Fresh tissue section without OCT embedding and H&E stain |
8 |
| Fresh tissue section with OCT embedding but without H&E stain |
8.1 |
| Fresh tissue scrape with OCT embedding and H&E stain |
7 |
| Tissue sections stored in −80°C for a day |
7.5 |
| Tissue sections stored in −80°C for a week |
6.5 |
| Tissue sections stored in −80°C for 1 month |
6 |
| Tissue sections stored in −80°C for 6 months |
2.2 |
| PTCs collected within 15 minutes after H&E staining |
6.2 |
| PTCs collected within 35 minutes after H&E staining | 1 |