Table 2.

Detection of viral RNA by qRT-PCR and IgG antibody titers to BoNoV in serum samples.

Calf #	PID (or age of negative control calf)	qRT-PCR resultsa		Serum IgG antibody titers
				VLPs of GIII.2 BoNoV (CV186-OH)		VLPs of GII.4 HuNoV (HS194)
				ELISA blocking solution Ab	ELISA blocking solution Bb	ELISA blocking solution A	ELISA blocking solution B
Infected
1	0	−	(<2.7)	<4	<4	<4	<4
	4	+	(4.1)	<4	<4	<4	<4
2	0	−	(<2.7)	<4	<4	<4	<4
	3	+	(4.0)	<4	<4	<4	<4
3	0	−	(<2.7)	<4	<4	<4	<4
	2	+	(4.1)	<4	<4	<4	<4
	9	−	(<2.7)	<4	1024	<4	<4
	30	−	(<2.7)	256	4096	<4	256
4	0	−	(<2.7)	<4	<4	<4	<4
	8	−	(<2.7)	<4	256	<4	<4
	21	−	(<2.7)	256	4096	<4	64
5	0	−	(<2.7)	<4	<4	<4	<4
	1	+	(5.8)	<4	<4	<4	<4
6	0	−	(<2.7)	<4	<4	<4	<4
	6	−	(<2.7)	<4	<4	<4	<4
7	0	−	(<2.7)	<4	<4	<4	<4
	1	−	(<2.7)	<4	<4	<4	<4
	5	+	(5.5)	<4	<4	<4	<4
Uninfected
8	13	−(<2.7)	<4	<4	<4	<4
9	8	−(<2.7)	<4	<4	<4	<4

^a+/−, real-time PCR-positive (viral RNA titers; log₁₀ GE per serum sample) or negative (<2.7 log₁₀ GE/ml; detection limit of the qRT-PCR for serum sample).

^bELISA blocking solution A; 2% non-fat dry milk (NFDM), ELISA blocking solution B; 1× buffered solution of casein (Universal Blocking Reagent, Biogenex, CA).