Skip to main content
. Author manuscript; available in PMC: 2014 Jan 29.
Published in final edited form as: Sci Transl Med. 2013 Jul 24;5(195):195ra94. doi: 10.1126/scitranslmed.3006448

Fig. 3. Suppressive activity of LDS Tregs.

Fig. 3

(A and B) The three subsets of Tregs (rTregs, CD45RA−Foxp3inter Tregs, and aTregs) were purified and cocultured at various ratios (1:1, 2:1, and 5:1) with CD4+ effector T cells that had been labeled with CellTrace Violet. Cultures were stimulated with anti-CD3 and irradiated antigen-presenting cells, and dilution of the dye, a marker of proliferation, was assessed 4 days later. (A) Results from a representative experiment (n = 3). (B) Percent suppression of effector T cell proliferation at the different ratios (median and range are indicated). LDS (n = 3) and nonallergic (NA) control (n = 3) Tregs effectively suppress effector T cell proliferation.