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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Chem Neuroanat. 2013 Oct 18;53:72–80. doi: 10.1016/j.jchemneu.2013.10.002

Figure 5.

Figure 5

Double immunofluorescence preparation to show that the pia mater is made up of glial fibers that co-contain ENaC γ-subunit and GFAP immunoreactivity. A. The ENaC γ-pia membrane surrounds the surface of the dorsal medulla, but it does not cover the dorsal surface of the area postrema (AP). One branch curves underneath the white matter associated with the gracile nucleus (Gr) and another branch travels ventral to nucleus tractus solitarius (NTS), ultimately defining the subpostremal NTS region (SubP). This plexus can be followed ventrally as midline raphe, dividing the NTS, and eventually surrounding the central canal. B. Dorsomedial part of the NTS co-contains immunoreactive ENaC γ-subunit and GFAP processes. C. The area ventral to the AP has glial processes that co-express ENaC γ-subunit and GFAP.