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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Immunol. 2013 Dec 9;192(1):41–51. doi: 10.4049/jimmunol.1301277

Figure 1. Additive effect of Munc18-2 and STX3 on mast cell degranulation.

Figure 1

A) Munc18-2 expression in RBL cells treated with indicated Munc18-2-specific siRNAs. The means of relative expression levels compared to scrambled siRNA are indicated below lanes (n=6). B) Effect of Munc18-2 silencing on β-hexosaminidase release after stimulation with IgE/Ag. Results are means ± SEM of 6 experiments. ** P < 0.01. C) Degree of silencing of Munc18-2, STX3 or both. One representative experiment (n=3) is shown. D) Effect of silencing of Munc18-2, STX3 or both on β-hexosaminidase release after stimulation with IgE/Ag or PMA/ionomycin. Results are means ± SEM of 3 experiments. * P < 0.05, ** P < 0.01. E) Effect of silencing Munc18-2, STX3 or both on IgE/Ag-induced CCL2 chemokine secretion. Released CCL2 at 4 h in cells treated with scrambled siRNA was arbitrarily set to 100 %. Results are means ± SEM of 3 experiments.