Table 1.
Subcellular distribution of Munc18-2 and Syntaxin3 in RPMC and effect of nocodazol and taxol treatment.
| Munc18-2 GP | Syntaxin3 GP | |||||
|---|---|---|---|---|---|---|
| Treatment | SG surface | SG matrix | Filamentous Connections | SG surface | SG matrix | Filamentous Connections |
| No treatment (25) | 13.8 ± 0.9a | 66.4 ± 5.0 | 19.9 ± 5.0 | 22.5 ± 2.7 | 68.1 ± 3.0 | 9.4 ± 2.1 |
| Nocodazole (17) |
26.9 ± 3.9b p < 0.001 |
67.9 ± 3.7 | 3.3 ± 1.6 |
38.8 ± 6.4 p < 0.02 |
54.9 ± 5.1 | 4.4 ± 2.2 |
| Taxol (16) |
29.4 ± 2.5 p = 0.001 |
66.0 ± 4.5 | 4.5 ± 1.4 | 31.4 ± 5.1 | 67.8 ± 5.1 |
0.8 ± 0.6 p < 0.02 |
a
Values are the mean (± SEM) of the % of Gold Particles (GP) in each subcellular compartment taking the total number of GPs in each picture as 100 %. The numbers of pictures scored is shown in parenthesis. Total GPs scored were at least 200 and 1500 for Syntaxin3 and Munc18-2, respectively
b
Numbers in bold indicate statistical significant differences as compared to untreated cells. Statistical analysis was carried out using GraphPAD Prism5 program. P was calculated using Student's t test for unpaired data, two tailed.