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. Author manuscript; available in PMC: 2014 Nov 12.

Published in final edited form as: Neuroscience. 2013 Jul 31;252:302–319. doi: 10.1016/j.neuroscience.2013.07.051

Fig. 11 — (A) RT-PCR analysis of TIMP-1: [F(3, 16)=1.45; P< 0.005] and TIMP-2 mRNA expression: [F(3, 16)=.63; P< 0.01] in WT, WT+aCSF, WT+Hcy and Hcy-treated WT+NaHS mouse brain. The GAPDH was using as loading control (B&C) Densitometry analysis of TIMP-1/TIMP-2 mRNA expressions as represented in the bar diagram. Data represents mean ±SE from n = 5 per group. ^**P< 0.005 vs Control group and ^##P< 0.001 vs Hcy treated group.

(D) Western blot analysis of TIMP-1: [F(3, 16)=.34; P< 0.001] and TIMP-2: [F(3, 16)=.55; P< 0.05] protein expression in mouse brain. The GAPDH was using as loading control (E & F) Densitometry analysis of TIMP-1/TIMP-2 protein expressions as represented in the bar diagram. Data represents mean ±SE from n = 5 per group. ^**P< 0.005 vs Control group and ^#P< 0.01, ^##P< 0.001 vs Hcy treated group.

Fig. 11 — (A) RT-PCR analysis of TIMP-1: [F(3, 16)=1.45; P< 0.005] and TIMP-2 mRNA expression: [F(3, 16)=.63; P< 0.01] in WT, WT+aCSF, WT+Hcy and Hcy-treated WT+NaHS mouse brain. The GAPDH was using as loading control (B&C) Densitometry analysis of TIMP-1/TIMP-2 mRNA expressions as represented in the bar diagram. Data represents mean ±SE from n = 5 per group. ^**P< 0.005 vs Control group and ^##P< 0.001 vs Hcy treated group.

(D) Western blot analysis of TIMP-1: [F(3, 16)=.34; P< 0.001] and TIMP-2: [F(3, 16)=.55; P< 0.05] protein expression in mouse brain. The GAPDH was using as loading control (E & F) Densitometry analysis of TIMP-1/TIMP-2 protein expressions as represented in the bar diagram. Data represents mean ±SE from n = 5 per group. ^**P< 0.005 vs Control group and ^#P< 0.01, ^##P< 0.001 vs Hcy treated group.