Table 1.
Genes | Orientation | Primers sequences |
---|---|---|
MMP9 | Forward Primers Reverse Primers |
5′-AAGGCAAACCCTGTGTGTTC-3′ 5′-GTGGTTCAGTT GTGGTGGTG-3′ |
MMP2 | Forward Primers Reverse Primers |
5′-GCACTCTGGAGCGAGGATAC -3′ 5′-GCCCTCCTAAGCCAGTCTCT-3′ |
iNOS | Forward Primers Reverse Primers |
5′-AACGGAGAACGTTGGATTTG-3′ 5′-CAGCACAAGGGGTTTTCTTC-3′ |
eNOS | Forward Primers Reverse Primers |
5′-CTGTGGTCTGGTGCTGGTC-3′ 5′-TGGGCAACTTGAAGAGTGTG-3′ |
ZO-1 | Forward Primers Reverse Primers |
5′-AAGGCAATTCCGTATCGTTG-3′ 5′-CCACAGCTGAAGGACTCACA-3′ |
Occuldin | Forward Primers Reverse Primers |
5′-GAGGGTACACAGACCCCAGA-3′ 5′-CAGGATTGCGCTGACTATGA-3′ |
TIMP1 | Forward Primers Reverse Primers |
5′-ACCATGGCCCCCTTTGAGCCCCTG-3′ 5′-TCAGGCTATCTGGGACCGCAGGGA-3′ |
TIMP2 | Forward Primers Reverse Primers |
5′-CTCGGCAGTGTGTGGGGTC-3′ 5′-CGAGAAACTCCTGCTTGGGG-3′ |
GFAP | Forward Primers Reverse Primers |
5′-CGAGTCCCTAGAGCGGCAAATG-3′ 5′-CGGATCTGGAGGTTGGAGAAAGTC-3′ |
TNFα | Forward Primers Reverse Primers |
5′-CCAGGAGAAAGTCAGCCTCCT-3′ 5′-TCATACCAGGGCTTGAGCTCA-3′ |
IL1-β | Forward Primers Reverse Primers |
5′-CACCTCTCAAGCAGAGCACAG-3′ 5′-GGGTTCCATGGTGAAGTCAAC-3′ |
GAPDH | Forward Primers Reverse Primers |
5′-TGAAGGTCGGTGTGAACGGATTTGGC-3′ 5′-CATGTAGGCCATGAGGTCCACCAC-3′ |