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. Author manuscript; available in PMC: 2014 Jan 29.
Published in final edited form as: Obesity (Silver Spring). 2012 Feb 9;20(7):1394–1402. doi: 10.1038/oby.2012.31

Figure 1.

Figure 1

Serum adropin levels in wild-type mice subjected to various nutritional challenges and in adropin knockout mice (AdrKO). (a) Adropin levels were measured using ELISA in sera collected from wild-type C57BL/6J (B6) mice that were fasted overnight or allowed ad libitum access to rodent chow. No adropin immunoreactivity was observed in sera collected from AdrKO. There were 5–6 mice per group, *P < 0.05 vs. fasted and AdrKO. (b) Measurement of serum adropin in lean B6 mice fed a purified low-fat diet (10% kJ/fat, 70% kJ/carbohydrate) or high-fat diet (60% kJ/fat, 20 kJ%/carbohydrate) for 48 h. All samples were collected at 0900 h from mice housed in a 12 h light:dark cycle, with lights-on from 0600–1800 h, with six mice per group, *P < 0.05 vs. the low-fat diet-fed mice.