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. Author manuscript; available in PMC: 2014 Jan 29.
Published in final edited form as: Obesity (Silver Spring). 2012 Feb 9;20(7):1394–1402. doi: 10.1038/oby.2012.31

Figure 2.

Figure 2

Gene targeting strategy used to generate adropin knockout mice (AdrKO). (a) The Enho gene is comprised of two exons, with the adropin open reading frame (ORF) situated in exon 2 (E2). A disrupted Enho allele was generated by inserting loxP sites into the single intron and 3′ of the adropin ORF in exon 2. Expression of Cre-recombinase removes part of the intron and the part of exon 2 containing the open reading frame. Note that this will also remove the neomycin selection cassette (Neo). (b) The transcript produced by the Enho gene is not detected by reverse transcription (RT)-PCR in total RNA samples from the liver, brain, and skeletal muscle of AdrKO. (c) RT-PCR analysis of Dnaic1 and Cntr expression in the central nervous system of WT (+/+), AdrHET (+/−), and AdrKO (−/−).