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Dihydroceramide desaturase 1 (DES) knockdown suppressed caspase 3-like (DEVDase) activation after photodynamic therapy (PDT). Following 2-h incubations post-PDT, cells were collected, lysed, and DEVDase activity was measured using Ac-DEVD-AMC as the fluorogenic substrate. The data are expressed as ratios of PDT-treated versus untreated controls and are shown as the mean±SEM, n=3-7. *DES knockdown suppresses PDT-induced DEVDase activation (p≤0.05).
