Skip to main content
. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Cell Signal. 2013 May 23;25(10):2047–2059. doi: 10.1016/j.cellsig.2013.05.012

Figure 4. CaMKI controls cell cycle progression in lung epithelia via p27 phosphorylation and its subcellular localization.

Figure 4

A549 cells were transfected with LacZ (A), CaMKI (B) or Fbxl12(C) plasmids followed by arrest at G0 by starvation. In 48 h 10% FBS was added and cells were collected at indicated times for flow cytometry and immunoblot analysis. The percentage of cells that entered into the S phase of the cell cycle are indicated at the top (S%). (D–G) CaMKI regulates p27 localization. MLE cells transfected with LacZ, CaMKI or Fbxl12 as described above were fixed and processed for immunostaining using p-CaMKI and p27 antibodies at indicated times. DAPI was used to visualize the nucleus. Scale bar, 10 μm. The data from each panel represents at least n=3 separate experiments.