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. Author manuscript; available in PMC: 2014 Jan 29.
Published in final edited form as: J Immunol. 2010 Sep 15;185(8):4602–4608. doi: 10.4049/jimmunol.1001892

Figure 3. Conjugation increases sensitivity of responding T cells to limiting amounts of antigen.

Figure 3

Mice injected with CFSE-labeled OTI T cells were challenged with the indicated amounts of the various forms of antigen as shown. Three days after transfer of OTI T cells, cells from the dLN were isolated and the OTI T cells assessed for the loss of CFSE as an indication of the presence of antigen in the host. The histograms shown were gated on live, CD8+, SIINFEKL-Kb tetramer+ events. The data shown are representative of three independent experiments.