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. 2014 Jan 9;14:7. doi: 10.1186/1471-2180-14-7

Table 3.

List of oligonucleotides used

Name Sequence (5’-3’)
pET16b_Fw
GCTAACGCAGTCAGGCACCGTGTA
pET16b_Rv
GACTCACTATAGGGGAATTGTGAGCG
tktC_Fw_XhoI
CCGGCTCGAGTTGTTTGATAAAATTGACCAT
tktC_Rv_XhoI
CCGGCTCGAGTTATTGTTTAAGTAAAGCT
tktP_Fw_XhoI
GCGCCTCGAGGTGCTCCAACAAAAAATAGAT CG
tktP_Rv_XhoI
GGCGCTCGAGTTAGAGAAGCTTTTTAAAATGAGAAA
tkt_C_Seq1
GCGTCATTTGGCAGCGGTATATAAT
tkt_C_Seq2
TCTAGGTCCTGAAGAACGAAAGC
tkt_C_Seq3
GGCTCGGCAGATCTTGCTAGTTC
tkt_P_Seq1
CCCTCATACGCTTTTTCAGAATC
tkt_P_Seq2
GCTAGAGCATTTAACACTGCACC
tkt_P_Seq3
CGATCTTGAACACTCTCACTAAATG
gapb_fw
GCGACTCGAGATGACCGTACGCGTAGCGATAA
gapb_rv GCGTCTCGAGTTACCTGAAAGCAACAGTAGC

Restriction sites are highlighted in italics, stop and start codons are underlined.