Table 3.
Name | Sequence (5’-3’) |
---|---|
pET16b_Fw |
GCTAACGCAGTCAGGCACCGTGTA |
pET16b_Rv |
GACTCACTATAGGGGAATTGTGAGCG |
tktC_Fw_XhoI |
CCGGCTCGAGTTGTTTGATAAAATTGACCAT |
tktC_Rv_XhoI |
CCGGCTCGAGTTATTGTTTAAGTAAAGCT |
tktP_Fw_XhoI |
GCGCCTCGAGGTGCTCCAACAAAAAATAGAT CG |
tktP_Rv_XhoI |
GGCGCTCGAGTTAGAGAAGCTTTTTAAAATGAGAAA |
tkt_C_Seq1 |
GCGTCATTTGGCAGCGGTATATAAT |
tkt_C_Seq2 |
TCTAGGTCCTGAAGAACGAAAGC |
tkt_C_Seq3 |
GGCTCGGCAGATCTTGCTAGTTC |
tkt_P_Seq1 |
CCCTCATACGCTTTTTCAGAATC |
tkt_P_Seq2 |
GCTAGAGCATTTAACACTGCACC |
tkt_P_Seq3 |
CGATCTTGAACACTCTCACTAAATG |
gapb_fw |
GCGACTCGAGATGACCGTACGCGTAGCGATAA |
gapb_rv | GCGTCTCGAGTTACCTGAAAGCAACAGTAGC |
Restriction sites are highlighted in italics, stop and start codons are underlined.