Table 1.
Neutralizing antibody titers of rhesus monkeys selected as SHIVIG donors
| RM |
Homologous clade C viruses |
Heterologous clade C viruses |
Heterologous clade B viruses |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SHIV-1157ip (early, tier 1) | SHIV-1157ipd3N4 (late, tier 2) | HIVZM135M | HIV1084i | HIVpIndieC | HIVZM233M.PB6 | HIVZM109F | SHIV-2873Nip | SHIVSF162P3 | HIVSF162.LS | HIVNL4-3 | SHIV89.6P | |
| RAo-8 |
2,048 |
>640 |
<20 |
|
128 |
42 |
<20 |
>640 |
<40 |
220 |
32 |
|
| RCt-10 |
360 |
>640 |
|
|
|
|
|
>640 |
|
6,500 |
|
|
| RHo-10 |
250 |
>640 |
|
|
|
|
|
>640 |
|
|
|
|
| RHy-9 |
600 |
>10,240 |
<20 |
100 |
90 |
<20 |
<20 |
>640 |
|
173 |
|
100 |
| RJa-9 |
1,800 |
>10,240 |
<20 |
|
128 |
59 |
22 |
2,048 |
68 |
35,770 |
128 |
|
| RLu-9 |
>1,280 |
>640 |
<20 |
50 |
47 |
<20 |
<20 |
>640 |
|
58 |
|
75 |
| RMf-9 |
2,048 |
>640 |
<20 |
|
128 |
<20 |
35 |
>640 |
78 |
18,303 |
128 |
|
| RPo-10 |
200 |
>640 |
|
|
|
|
|
>640 |
|
|
|
|
| RTs-7 | 2,048 | >640 | 128 | >640 | 32 | |||||||
NAb titers (reciprocal dilution of sera giving 50% reduction in virus replication) were determined in TZM-bl cells or, for SHIV-1157ipd3N4 and SHIV-2873Nip, in human PBMC-based assays. The SHIVs were prepared in RM PBMC; HIV1084i, HIVNL4-3 and HIVpIndieC were prepared in human PBMC; nAb titers against the remaining HIV strains were performed using pseudoviruses generated in transfected 293 T cells. All neutralization assays were performed at least in duplicate.