Figure 3.

Effect of UVB on CL1-0 and CL1-0ΔGR ROS production. Microtiter plate-based DCFH analysis of intracellular ROS production assays were performed where CL1-0 and CL1-0∆GR cells (50,000 cells / well) were plated into 24-well plates. After 24 h, the cells were treated with 45 mJ/cm² of UVB. Cells were further incubated for indicated periods before treated with 10 μM of DCFH-DA at 37°Cfor 20 min and the fluorescence was recorded at excitation and emission wavelengths of 485 nm and 530 nm, respectively. Data were represented as mean ± SD (p < 0.05*, p < 0.01**, p < 0.001 ***).