Table 1. Summary of high-throughput mass spectrometry analysis of miRNA-precipitated proteins.
| CLmiRNA | EXOmiRNA | Poly-A | Beads | |
|---|---|---|---|---|
| hnRNPA2B1 | 0 | 4 | 0 | 0 |
| hnRNPA1 | 0 | 2 | 0 | 0 |
| hnRNPC | 0 | 1 | 0 | 0 |
| hnRNPU | 1 | 3 | 0 | 0 |
| RS4X | 0 | 3 | 0 | 0 |
| RS3 | 1 | 3 | 0 | 0 |
| NUCL | 6 | 9 | 8 | 0 |
CLmiRNA, miRNA sorted into cells, EXOmiRNA, miRNA sorted into exosomes; hnRNPA2B1, heterogeneous nuclear ribonucleoprotein A2B1; hnRNPA1, heterogeneous nuclear ribonucleoprotein A1; hnRNPC, heterogeneous nuclear ribonucleoprotein C; hnRNPU, heterogeneous nuclear ribonucleoprotein U; RS4X, 40S ribosomal protein S4; RS3, 40S ribosomal protein S3; NUCL, nucleolin; miRNA, micro RNA.
Exosome extracts were incubated with streptavidin beads coated with either a biotinylated EXOmiRNA (miR-198), a biotinylated CLmiRNA (miR-17) or a negative control (poly-A), and with non-coated beads (beads). The table represents the number of peptides of hnRNPA2B1, hnRNPA1 and other related proteins that were identified in the precipitated fractions.