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. 2013 Nov 14;4:2787. doi: 10.1038/ncomms3787

Figure 5. Activation of the Wnt pathway assayed by the SuperTopFlash reporter.

Figure 5

(a–f) Co-transfected decreasing doses (25, 5 and 1 ng) of His-tagged R-spondin constructs used for SPR experiments in Fig. 4. Error bars represent s.d. from three replicates. (g,h) Western blots showing expression levels of the His-tagged R-spondin constructs from whole-cell lysate and conditioned media (CM). Expression for mRspo2 Fu1-His was poor and below the level of detection; however, the individual Fu1 domain from RSPO1 was detected by western blotting and produced identical results (Supplementary Fig. S1b,c). RLU, relative luciferase units.