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. 2014 Jan;14(1):69–95. doi: 10.2174/15665240113136660079

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© 2013 Bentham Science Publishers

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. (12) — Anti-p200 pemphigoid (a) Erythema, blisters, erosions and crusts in a 53-year old patient with anti-p200 pemphigoid. (b) Histopathological examination reveals subepidermal cleavage and a neutrophil-rich inflammatory infiltrate. (c) Direct immunofluorescence (IF) microscopy analysis of perilesional skin shows linear IgG deposition at the dermo-epidermal junction. (d) Serum IgG autoantibodies bind to the dermal side of 1M NaCl-split skin by indirect IF microscopy (all magnification 200x). (e) Dermal extracts were separated by 6% SDS-PAGE, transferred on nitrocellulose and immunoblotted with serum from patients with epidermolysis bullosa acquisita (EBA; lane 1), anti-p200 pemphigoid (p200; lane 2) and normal human serum (NHS, lane 3).