Figure 6. Fe-deficiency tolerance and Fe accumulation in the HRZ insertion and complementation lines.

(a and b) Fe-deficiency tolerance in hydroponic culture. The SPAD value of the newest leaves was measured after the onset of Fe-deficiency treatment. Means±s.d. of four to 15 biological replicates. (c) Fe concentrations in leaves subjected to Fe-deficient hydroponic treatment for 7 days. (d) Fe concentrations in brown seeds from pot culture in normal soil. Fe concentrations are shown as means±s.d. of μg g⁻¹ DW of two to eight replicates derived from two to four biological replicates. WT, wild-type cultivar DongJing for hrz1-1 and horz1-1, and cultivar Nipponbare for hrz2-1 and the complementation lines. The line numbers of the hrz2-1-HRZ2 and hrz2-1-HRZ1 complementation lines are shown as numerals. Asterisks indicate significant differences compared with WT (two-sample Student’s t-test; *P<0.05; **P<0.01; Supplementary Table S1).