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. 2014 Jan 20;5:3164. doi: 10.1038/ncomms4164

Figure 2. Maspin knockout mice are ostensibly normal.

Figure 2

(a) Comparison of maspin mRNA in adult wild-type (wt) and Cre-deleted knockout (ko) mice. Reverse transcriptase PCR analysis demonstrates products of predicted size (wt: 602 bp; ko: 455 bp), validated by DNA sequencing (see Fig. 1d). (b) ko mice do not produce maspin protein, as assessed by immunoblotting using the anti-human maspin monoclonal antibody 13G11 or rabbit anti-peptide antibody H130. Validation of these antibodies is shown in Supplementary Fig. 1. (c) Maspin ko skin lacks maspin in epidermal cells, as assessed by immunohistochemistry of samples from 8-week old mice, using 13G11. Scale bar, 30 μm.