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. Author manuscript; available in PMC: 2014 Jun 21.
Published in final edited form as: Circ Res. 2013 Apr 23;113(1):e1–e9. doi: 10.1161/CIRCRESAHA.113.301112

Figure 3. Characterization of pegylated human rHDL.

Figure 3

(A) rHDL pegylated with M-PEG-ALD 20K at 4°C for 16 hours and analyzed by SDS-PAGE and Coomassie Blue staining. (B) Cholesterol efflux for 3 hours from cholesterol-loaded LXR activated THP-1 macrophages to 20 or 30 μg rHDL or PEG-rHDL protein/ml. (C) Plasma clearance of rHDL or PEG-rHDL following injection of 60mg/kg rHDL or PEG-rHDL protein into circulation and analyzed by Western analysis. N.S.= non-specific band. (D) Densitometry quantification and analysis of (C), n=4.