Figure 6.
PHQS strand bias correlates with the potential of PHQS to form HQ. (A) Formation of stable HQ requires a minimum of two G3 tracts. DNA bearing the indicated G-core downstream of a T7 promoter (T7P) was subjected to no treatment (−), or a heat denaturation/renaturation (H), or transcription with T7 polymerase and GTP or dzGTP. HQ formation was detected by native gel electrophoresis. Asterisk indicates the band of DNA containing HQ and its amount as percent of the total DNA in the lane. (B) PHQS Strand Bias in 61 species in the Ensembl database. The species is ordered according to the species tree provided on the Ensembl Web site. (C) Occurrence of isolated G3 tracts in the ±4-kb region around TSS in human, chicken and stickleback. Red and green curves indicate non-template and template DNA strand, respectively.