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. 2013 Sep 9;41(22):10135–10149. doi: 10.1093/nar/gkt770

Figure 4.

Figure 4.

UBF1 N-terminal domain is sufficient to rescue viability of rpa49Δ-hmo1Δ strain. (A) Schematic representation of UBF1. UBF1 depicted as an N-terminal dimerization domain (UBF-D) and five HMG Boxes (1–5). Truncated UBF1 constructions, Box1 and Box1-2, are shown by square brackets. (B) YFP-truncated proteins were co-expressed with CFP-Nup49 and mRFP-Nop1 fusion proteins that, respectively, delineate the nuclear periphery (blue) and the nucleolus (red). Truncated UBF1 constructions Box1 and Box1-2 localized in the yeast nucleolus. (C) UBF1-Box1-2 is sufficient to stimulate Pol I. Ten-fold serial dilutions of the rpa49Δ-hmo1Δ strain expressing the UBF1 truncation Box1, Box1-2, or Hmo1 or an empty vector (−) were spotted onto plates containing 5-FOA to test for complementation (see ‘Materials and Methods’ section).