Skip to main content
. 2013 Aug 31;41(22):10086–10109. doi: 10.1093/nar/gkt777

Figure 6.

Figure 6.

Small RNAs activate transcription through a distinct pathway. (A) qPCR data showing COX-2 mRNA expression following transfection (TRF) of either mismatched RNA (MM) or RNA12 (25 nM) in combination with either vehicle control, or activators of COX-2 expression (IL1β, 10 ng/ml; TNFα, 100 ng/ml; PMA, 50 ng/ml). Numbers indicate fold activation relative to MM. Compound was added to cells 2 days after transfection of RNA and removed after 24 h. n = 3. (B) qPCR showing COX-2 mRNA levels following treatment with PMA (50 ng/ml), IL1β (10 ng/ml), TNFα (100 ng/ml) or RNA12 (25 nM) alone, or in combination with Dex (1 µM). Vehicle = control. Cells were treated with compound for 24 h. n = 2. Error bars are SD. ***P < 0.001 (t-test) relative to MM.