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. 2013 Sep 5;41(22):10476–10487. doi: 10.1093/nar/gkt799

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Calculation of and via RP-HPLC analysis. (A) HPLC profile of 3′-^SeUMP at 260 nm (solid line) and 370 nm (dash line). (B) HPLC profile of 5′-^SeUU-3′ dimer at 260 nm (solid line) and 370 nm (dash line). The samples (3′-^SeUMP and 5′-^SeUU-3′) were analyzed on a Welchrom XB-C18 column (4.6 × 250 mm, 5 μ) at a ﬂow of 1.0 ml/min and with a linear gradient of 5–50% B in 20 min, with a retention time of 10.3 and 13.6 min, respectively. Buffer A: 10 mM TEAAc (pH 7.1); B: 50% acetonitrile in 10 mM TEAAc (pH 7.1).

Inline graphic — Calculation of and via RP-HPLC analysis. (A) HPLC profile of 3′-^SeUMP at 260 nm (solid line) and 370 nm (dash line). (B) HPLC profile of 5′-^SeUU-3′ dimer at 260 nm (solid line) and 370 nm (dash line). The samples (3′-^SeUMP and 5′-^SeUU-3′) were analyzed on a Welchrom XB-C18 column (4.6 × 250 mm, 5 μ) at a ﬂow of 1.0 ml/min and with a linear gradient of 5–50% B in 20 min, with a retention time of 10.3 and 13.6 min, respectively. Buffer A: 10 mM TEAAc (pH 7.1); B: 50% acetonitrile in 10 mM TEAAc (pH 7.1).