Table 1.
MALDI-TOF-MS Analysis of SeU-RNA
| Entry | Se-RNAs | Measured (calcd) m/z |
|---|---|---|
| 1 | 5′-U-SeU-CGCG-3′ (C56H71N20O41P5Se) | [M + H]+: 1915.4 (1915.2) |
| 2 | 5′-G-SeU-GUACAC-3′ (C76H95N30O53P7Se) | [M + H]+: 2573.3 (2573.3) |
| 3 | 5′-GUG-SeU-ACAC-3′ (C76H95N30O53P7Se) | [M + H]+: 2573.5 (2573.3) |
| 4 | 5′-AUGG-SeU-GCUC-3′ (C85H106N32O62P8Se) | [M + H]+: 2895.3 (2895.7) |
| 5 | 5′-CGCGAA-SeU-UCGCG-3′ (C114H144N46O81P11Se) | [M + H]+: 3873.3 (3874.5) |
| 6 | 5′-CGCGAAU-SeU-CGCG-3′ (C114H144N46O81P11Se) | [M + H]+: 3874.0 (3874.3) |
| 7 | 5′-U-SeU-AUAUAUAUAUAA-3′ (C133H162N49O95P13Se) | [M + H]+: 4449.7 (4449.6) |
| 8 | 5′-AA-SeU-A(2′-SeMe-U)AUAUAUAUU-3′ (C134H164N49O94P13Se2) | [M + H]+: 4526.4 (4526.4) |
| 9 | 5′-GG-SeU-AUUGCGGUACC-3′ (C133H165N52O97P13Se) | [M + H]+: 4526.4 (4526.7) |
| 10 | 5′-A-SeU-CACCUCCUUA-3′ (C111H141N38O82P11Se) | [M + H]+: 3740.8 (3740.2) |
| 11 | U-SeU-AGCUAGCU (C94H117N34O69P9Se) | [M + H]+: 3186.2 (3185.9) |
| 12 | U-SeU-CGCGAUCGCG (C113H142N43O83P11Se) | [M + H]+: 3851.7 (3851.3) |
| 13 | U-SeU-CAUGUGACC (C103H129N37O76P10Se) | [M + H]+: 3489.8 (3490.4) |