Table 2.

UV-melting temperatures of ^SeU-RNAs

Entry	Modified region	RNA sequences	Tm (°C)	ΔTm (°C)
1		(5′-rUUAUAUAUAUAUAA-3′)2	38.0 ± 0.3
2	Duplex	(5′-rUUA-SeU-AUAUAUAUAA-3′)2	37.3 ± 0.5	−0.7
3		(5′-rGGUAUUGCGGUACC-3′)2	45.0 ± 0.4
4	Duplex	(5′-rGG-SeU-AUUGCGGUACC-3′)2	44.2 ± 0.3	−0.8
5		(5′-rCGCGAAUUCGCG-3′)2	39.4 ± 0.4
6	Duplex	(5′-rCGCGAAU-SeU-CGCG-3′)2	39.0 ± 0.3	−0.4
7		5′-AUCACCUCCUUA-3′	43.2 ± 0.3
		3′-UAGUGGAGGAAU-5′
8	Duplex	5′-A-SeU-CACCUCCUUA-3′	42.8 ± 0.4	−0.4
		3′-U–A–GUGGAGGAAU-5′
9		(5′-UUAGCUAGCU-3′)2	42.2 ± 0.2
10	Duplex junction	(5′-U-SeU-AGCUAGCU-3′)2	47.1 ± 0.3	+4.9
11		(5′-UUCGCGAUCGCG-3′)2	59.4 ± 0.3
12	Duplex junction	(5′-U-SeU-CGCGAUCGCG-3′)2	63.2 ± 0.3	+3.8
13		5′-UUCAUGUGACC-3′	48.2 ± 0.3
		3′—–GUACACUGGUU-5′
14	Duplex junction	5′-U-SeU-CAUGUGACC-3′	49.9 ± 0.4	+1.7
		3′——–GUACACUGGUU-5′