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. 2013 Sep 9;41(22):10157–10169. doi: 10.1093/nar/gkt812

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — p18CycE interferes with chromatin retention of NHEJ components. (A) Nuclear extracts of HEK 293T cells stably expressing p18CycE or EGFP were used for determining the levels of NHEJ proteins, XRCC4, Lig IV, XLF, Ku70-80 and DNA-PKcs recruited to DNA-conjugated beads. Input was used to indicate equal protein lysates. Time-course for recruitment of NHEJ proteins to the chromatin fraction of parental and p18CycE-expressing derivative (B) HEK 293T kidney and (D) C4-2 prostate cancer cells. (C) DNA-PKcs autophosphorylation and levels of DNA-PKcs and 53BP1 in the chromatin-bound fraction of HEK 293T parental and p18CycE-expressing derivative cells. Histone H4 and PARP-1 were used as loading controls.