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. 2013 Sep 7;41(22):10641–10658. doi: 10.1093/nar/gkt801

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Smoothed and normalized UV absorbance data while annealing (at 200 nM). The lower temperature transition is the (bimolecular) formation of the complex, whereas the higher temperature transition is the (unimolecular) formation of the hairpin. The mean absorbance between and 35°C is normalized to 0 and that between and 66°C (indicated by vertical lines) to 1. The temperature range whose mean absorbance is normalized to 1 is concentration-dependent (Supplementary Table S3). Data acquired by annealing and melting are essentially superimposable. The dashed line indicates the halfway point of the bimolecular transition.

Inline graphic — Smoothed and normalized UV absorbance data while annealing (at 200 nM). The lower temperature transition is the (bimolecular) formation of the complex, whereas the higher temperature transition is the (unimolecular) formation of the hairpin. The mean absorbance between and 35°C is normalized to 0 and that between and 66°C (indicated by vertical lines) to 1. The temperature range whose mean absorbance is normalized to 1 is concentration-dependent (Supplementary Table S3). Data acquired by annealing and melting are essentially superimposable. The dashed line indicates the halfway point of the bimolecular transition.