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. 2013 Aug 30;41(22):10268–10282. doi: 10.1093/nar/gkt775

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Depletion of TRF1 impairs the formation of IR-induced (pT371)TRF1 foci. (A) Western analysis of TRF1 depletion in HeLaII cells. Immunoblotting was performed with anti-pT371 or anti-TRF1 antibody. The γ-tubulin blot was used as a loading control in this and the following experiments. (B) Western analysis of shTRF1-resistant Myc-tagged wild-type or mutant TRF1 proteins in TRF1-depleted HeLaII cells. (C) Indirect IF with anti-pT371 antibody. HeLaII cells expressing various constructs as indicated were treated with 12 Gy IR. Cell nuclei were stained with DAPI in blue. (D) Quantification of the percentage of cells containing five or more IR-induced (pT371)TRF1 foci from (C). Standard deviations from six independent experiments are indicated.