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. 2013 Aug 30;41(22):10268–10282. doi: 10.1093/nar/gkt775

Figure 5.

Figure 5.

The lack of TRF1 phosphorylation at T371 impairs the activation of the G2/M checkpoint and hampers the maintenance of genome integrity. (A) Quantification of the percentage of cells stained positive for H3-pS10. For each cell line, 3000 cells were scored in blind. Standard deviations from four independent experiments are indicated. (B) Quantification of the percentage of chromosome/chromatid breaks and gaps per metaphase cell. TRF1-depleted HeLaII cells stably expressing the vector alone or various TRF1 alleles as indicated were mock treated or treated with 1 Gy IR. For each cell line, 60 metaphase cells were scored in blind. Standard deviations from three independent experiments are indicated. (C and D) Clonogenic survival assays following various doses of CPT (C) or IR (D). The colony forming assays were performed with TRF1-depleted HeLaII cells stably expressing the vector alone or various TRF1 alleles as indicated. HeLaII cells expressing both pRS and pWZL vectors were also included as a control. Standard deviations from six independent experiments are indicated.