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. 2013 Sep 3;41(22):10462–10475. doi: 10.1093/nar/gkt798

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Structural comparison of the Bsu and Tte preQ₁ riboswitches. (A) Structure of preQ₁ (7-aminomethyl-7-deazaguanine). (B) Structural overlay of the Bsu (colored, PDB ID 3FU2, chain A) and Tte (gray, PDB ID 3Q50) riboswitch crystal structures. The sugar-phosphate backbone is shown as a single ribbon. preQ₁ is space-filled and colored as in A. Secondary structure elements are color-coded as indicated. (C) Secondary structure maps of the Bsu and Tte riboswitches with interactions shown in Leontis–Westhof nomenclature (14). Individual secondary structures are color-coded as in (B), and the locations of fluorophores and biotin are indicated. (D) Prism-based TIRFM setup for smFRET.