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. 2013 Sep 3;41(22):10462–10475. doi: 10.1093/nar/gkt798

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effect of ligand on the distribution of the mid- and high-FRET states. (A) The FRET histograms of Figure 2 were quantified, and the percentage high-FRET state was plotted as a function of ligand concentration. The data were fit with a non-cooperative binding isotherm and the respective apparent K_1/2 values are indicated for both Bsu (closed symbols) and Tte (open symbols). (B) The centers of the Gaussian fits for the mid-FRET (green) and high-FRET (blue) states from Figure 2 were plotted as a function of ligand concentration and fit with a non-cooperative binding isotherm, yielding the K_1/2 values indicated for the Tte riboswitch.