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. 2013 Sep 14;41(22):10334–10344. doi: 10.1093/nar/gkt833

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — RPA colocalizes with 53BP1 and ATR in G1 phase. (A) IR-induced ATR foci colocalize with RPA foci. GFP-ATR expressing U2OS cells in G1 phase were treated with 2 Gy IR and fixed after 75 min. Cells were immunostained with antibodies against RPA2. (B) Asynchronous GFP-53BP1 expressing U2OS cells were treated with 2 Gy IR and fixed after 75 min. Cells were co-immunostained with antibodies against RPA2 (detected by anti-mouse-Alexafluor647) and the cell cycle markers cyclin D1, geminin or cyclin A (detected by anti-rabbit-Cy3). 134 × 287 mm (300 × 300 DPI).