Figure 3.

Growth characteristics of the parE mutant and the complementation strain. (A) 3456, 3456ΔparE, and 3456ΔparE/pIJ82–parE were grown on SFM agar for 4 days at 30°C. 3456ΔparE remained white with aerial hyphae, while the other two strains had produced gray spores. (B) 3456, 3456ΔparE, and 3456ΔparE/pIJ82–parE were grown over coverslips on MM containing mannitol for 13 days, and the spores were collected from the coverslips, stained with DAPI and imaged under a fluorescence microscope. Image contrast has been increased for better clarity. More sample photos are in Supplementary Figure S2.