. 2013 Aug 31;41(22):10403–10413. doi: 10.1093/nar/gkt757

Table 3.

The parE knockout mutant can maintain the linear plasmid (pLUS356L) but not circular plasmid (pLUS355)

Treatment	3456^a	3456ΔparE^a	Total^a
No. cfu regenerated	2.3 × 10⁶	5.2 × 10⁶	7.5 × 10⁶
No. pLUS355 transformants	594	0	594
No. pLUS356/AseI transformants	221	120^b	341

^aTotal transformants were scored by thiostrepton resistance; 3456ΔparE was scored by the number of Apr^r transformants; 3456 was scored by subtracting the total number of transformants by that of Apr^r transformants.

^bTwenty-two transformants were checked by Southern hybridization, and all showed the existence of the linear plasmids.