Table 1.
Parameters determined from analysis
| DNA | Tm (°C) | P (nm) | PManning (nm) | γ0 (base/turn) | C (× 10−19 erg-cm) | Pt (nm) |
|---|---|---|---|---|---|---|
| 1 | 87.1 ± 0.5 | 51.5 ± 0.2 | 51.5 | 10.52 ± 0.01 | 2.27 ± 0.11 | 55.7 ± 2.6 |
| 2 | 85.3 ± 0.6 | 45.4 ± 0.4 | – | 10.47 ± 0.01 | 2.30 ± 0.13 | 56.5 ± 3.1 |
| 3 | 93.3 ± 0.7 | 42.9 ± 0.3 | – | 10.62 ± 0.01 | 3.07 ± 0.19 | 75.4 ± 4.7 |
| 4 | 91.5 ± 0.7 | 44.3 ± 0.1 | 38.1 | 10.62 ± 0.01 | 1.48 ± 0.05 | 36.4 ± 1.2 |
| 5 | 88.3 ± 0.3 | 41.8 ± 0.1 | – | 10.69 ± 0.01 | 0.85 ± 0.04 | 20.9 ± 1.0 |
| 6 | 86.5 ± 0.7 | 41.8 ± 0.1 | 64.6 | 10.51 ± 0.02 | 0.61 ± 0.03 | 14.9 ± 0.8 |
| 7 | 93.4 ± 0.3 | 50.3 ± 0.1 | – | 10.64 ± 0.01 | 1.09 ± 0.07 | 26.8 ± 1.6 |
| 8 | 86.8 ± 0.4 | 58.5 ± 0.2 | 58.1 | 10.57 ± 0.01 | 1.08 ± 0.08 | 26.5 ± 1.9 |
| 9 | 93.0 ± 0.5 | 49.5 ± 0.2 | – | 10.58 ± 0.01 | 3.24 ± 0.07 | 79.6 ± 1.8 |
The reported values of Tm from thermal denaturation of modified DNAs are the mean and standard deviation from three independent repeats. P, γ0 and C along with the related Pt are presented as mean ± standard deviation from Monte Carlo simulations. Manning’s theoretical charge-stiffness prediction for persistence length (PManning) is shown for comparison (11).