Table 1.
16S rDNA-based identification of a collection of 47 “difficult-to-identify” bacteria clinical isolates
|
Genus/strain |
No. of isolates |
Source of isolates |
GenBank accession no. |
BLAST |
|
|---|---|---|---|---|---|
| E-value | % ID | ||||
| Gram-negative |
22 |
|
|
|
|
|
Acinetobacter johnsonii |
1 |
Secretion |
KF381212 |
0.0 |
100 |
|
Aggregatibacter aphrophilus |
1 |
Blood |
KF410885 |
0.0 |
100 |
|
Agrobacterium tumefaciensa |
1 |
Blood |
JF487939 |
0.0 |
100 |
|
Bacteroides fragilis |
1 |
Blood |
KF381209 |
0.0 |
100 |
|
Bilophila wadsworthiaa, |
1 |
Pleural fluid |
KF414628 |
0.0 |
99 |
|
Campylobacter fetus |
1 |
Blood |
KF372434 |
0.0 |
100 |
|
Capnocytophaga sputigenaa |
3 |
Blood, secretion |
JF422019 |
0.0 |
99 |
|
Delftia sp. |
1 |
Puncture fluid |
KF410887 |
0.0 |
--c |
|
Dialister pneumosintesa |
1 |
Cerebrospinal fluid |
KF410890 |
0.0 |
100 |
|
Eikenella corrodens |
1 |
Blood |
KF372435 |
0.0 |
100 |
|
Enterobacter sp. |
1 |
Blood |
KC148534 |
0.0 |
--c |
|
Haemophilus influenzae |
1 |
Blood |
JF487936 |
0.0 |
100 |
|
Massilia sp.a |
1 |
Puncture fluid |
KF410886 |
0.0 |
100 |
|
Moraxella osloensis |
3 |
Blood, secretion |
KF372432 |
0.0 |
100 |
|
Neisseria meningitidis |
2 |
Cerebrospinal fluid, blood |
KF372431 |
0.0 |
99 |
|
Pontibacter sp.b |
1 |
Puncture fluid |
KF410889 |
0.0 |
--c |
|
Prevotella bivia |
1 |
Secretion |
KF372433 |
0.0 |
99 |
| Gram-positive |
23 |
|
|
|
|
|
Bacillus amyloliquefaciens |
1 |
Puncture fluid |
KF410881 |
0.0 |
99 |
|
Bacillus niabensisb |
1 |
Puncture fluid |
KF381217 |
0.0 |
100 |
|
Bacillus pumilus |
1 |
Secretion |
KF381216 |
0.0 |
99 |
|
Brachybacterium sp. |
1 |
Secretion |
KF410892 |
0.0 |
--c |
|
Corynebacterium amycolatum |
1 |
Blood |
JF487935 |
0.0 |
99 |
|
Corynebacterium falsenii |
1 |
Blood |
KF414626 |
0.0 |
100 |
|
Corynebacterium sp. |
2 |
Blood, secretion |
KF414627 |
0.0 |
--c |
|
Corynebacterium xerosis |
1 |
Blood |
KF410882 |
0.0 |
100 |
|
Granulicatella adiacens |
1 |
Blood |
KF381213 |
0.0 |
100 |
|
Janibacter sp.a |
1 |
Blood |
KF410884 |
0.0 |
--c |
|
Leifsonia sp.a |
2 |
Blood |
KF410883 |
0.0 |
--c |
|
Nocardia sp. |
6 |
Secretion |
KF381210 |
0.0 |
--c |
|
Paenibacillus massiliensisa |
1 |
Puncture fluid |
KF381215 |
0.0 |
100 |
|
Propionibacterium acnes |
1 |
Blood |
KF381211 |
0.0 |
100 |
|
Rhodococcus equi |
1 |
Secretion |
KF410888 |
0.0 |
100 |
|
Streptomyces sp. |
1 |
Secretion |
KF410891 |
0.0 |
--c |
| Acid-fast staining positived |
2 |
|
|
|
|
|
Mycobacterium abscessus |
1 |
Secretion |
JF487938 |
0.0 |
100 |
| Mycobacterium tuberculosis | 1 | Sputum | KF410893 | 0.0 | 100 |
aThe clinical isolates first reported in China.
bThe clinical isolates first reported in the international literature.
cMore than two species in the same genus with the % ID ≥ 99%.
dAll these isolates are not available to be identified by Phoneix or Microscan, and exception of these were attempted to identify by Phoneix.