Table 2. Nasal shedding of striped skunks (Mephitis mephitis) experimentally infected with an avian influenza virus (H4N6).
| DPI | |||||||||||||||||
| Animal | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 16 | 20 |
| A | --a | 2.90*b | 4.70* | 4.61* | 5.14* | 5.65* | 4.85* | 5.51* | 5.70* | 4.73* | 4.02* | 3.54 | 4.24 | 4.45 | 3.03 | 1.85 | Sc |
| B | -- | 3.15* | 4.15* | 4.58* | 4.51* | 4.89* | 4.94* | 5.19* | 6.02* | 4.48* | 3.67* | 3.35 | 2.44 | 3.36 | S | nt | S |
| C | -- | 1.73 | 3.82* | 3.39* | 3.69* | 4.33* | 5.00* | 4.65* | 5.42* | 4.72* | 4.15* | 3.99 | 2.38 | 1.96 | S | nt | -- |
| D | -- | 2.92* | 4.11* | 4.79* | 4.83* | 4.98* | 4.97* | 4.97* | 5.42* | 3.56* | S* | S | S | 2.13 | -- | nt | -- |
| E | -- | S | S | 2.75* | 2.50* | 3.91* | 2.17nt | 3.78* | 3.72* | 3.37* | 3.12* | S | 2.10 | -- | -- | nt | -- |
| F | -- | 3.21* | 4.44* | 4.20* | 4.07* | 4.56* | 4.13* | 4.99* | 5.12* | 4.35* | 3.51* | S | S | S | -- | nt | -- |
| G | -- | 2.69* | 3.24* | 4.04* | 3.85* | 4.47* | 4.72* | 4.64* | 5.97* | 4.55* | 3.88* | 3.65 | S | S | -- | nt | -- |
| Controld | -- | -- | -- | -- | -- | -- | -- | -- | -- | -- | -- | -- | -- | -- | -- | nt | |
a
Nasal shedding was assessed via nasal washes by RRT-PCR. Results are presented as log10 PCR EID50 equivalents/mL. A dash “--” indicates that no viral RNA was detected. The term “nt” indicates the sample was not taken or there was insufficient sample volume to conduct the test.
b
* = Live virus confirmed by virus isolation in eggs. Only samples from 1–10 DPI were tested.
c
Suspect positive: two wells positive but Ct>38.
d
Individual was a mock inoculated control skunk housed in the same animal room, but in a separate pen.