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. 2014 Jan 29;9(1):e86366. doi: 10.1371/journal.pone.0086366

Figure 4. ABX treatments protected PD- and TD-fed mice from DSS-induced colitis.

Figure 4

WT mice were treated continuously with ABX and fed PD or TD for 2% DSS treatment. (A) Percent original BW of ABX treated PD- or TD-fed mice following the start of DSS treatment (n = 3–4 mice/group). Significant difference in BW change was found in PD versus TD, PD versus PD ABX, TD versus TD ABX, and PD ABX versus TD ABX (***P<0.001, two-way ANOVA). (B) Colonic length at d0 and d5 following the start of DSS (n = 3–4 mice/group and time point). There was a significant effect of DSS treatment over time and between groups as indicated (*P<0.05, **P<0.01, ***P<0.001, two-way ANOVA with Bonferroni post-tests). (C) Histological scores of distal colon at d5 post DSS (n = 3–4 mice/group). TD was significantly different from PD and TD ABX (**P<0.01, ***P<0.001, one-way ANOVA with Tukey's post-tests). Data is from one representative of three experiments. (D) Blood scores from PD- and TD-fed conventional or germ-free mice following 5d of DSS treatment (n = 3–6 mice/group). TD was significantly different from PD in conventionally raised mice (**P<0.01, two-way ANOVA with Bonferroni post-tests). Data shown are the mean +/− SEM. ABX, antibiotics; BW, body weight; DSS, dextran sodium sulfate; PD, purified diet; TD, Teklad diet; WT, wild-type.