Table 2. Polymorphism characteristics of eight microsatellite loci genotyped in 162 F0 zebra finch individuals.

Locus	EMBL accession number	Chromosome	Reference	Fluorescent label used	Multiplex	Number of alleles	Allelic size range (bp)	Ho	He
Tgu 1	EF090891	Tgu5	Forstmeier et al. [45]	FAM	1	13	165–218	0.723	0.848
Tgu 3	EF090892	Tgu9	Forstmeier et al. [45]	VIC	1	15	146–182	0.792	0.881
Tgu 4	EF090893	Tgu6	Forstmeier et al. [45]	FAM	2	20	87–137	0.881	0.913
Tgu 5	EF090894	Tgu1A	Forstmeier et al. [45]	NED	2	15	143–185	0.570	0.867
Tgu 8	EF090897	Tgu2	Forstmeier et al. [45]	FAM	2	16	180–228	0.841	0.909
Tgu 9	EF090898	Tgu2	Forstmeier et al. [45]	NED	1	13	127–164	0.796	0.882
Tgu 12	EF090901	Tgu1	Forstmeier et al. [45]	NED	2	15	94–120	0.804	0.880
Indigo 41	AF361047	Tgu2	Dawson et al. [46]	FAM	1	19	292–442	0.830	0.911

Ho, observed heterozygosity; He, expected heterozygosity. All of the loci deviated significantly from HWE at P<0.0001.