Figure 2. Magnesium dependence of D-RNA hydrolysis by hammerhead Spiegelzymes.

A. D-RNA (0.2 µM) was incubated with 2 µM of the Spiegelzyme in 50 mM Tris-HCl, pH 7.5, buffer containing 1, 5, 10, and 25 mM MgCl₂ for 1 h at 37°C. The reaction products were separated by 20% polyacrylamide gel electrophoresis with 7 M urea. Control reactions were carried out with target D-RNA alone in buffer (lane Control A) or with D-RNA hammerhead ribozyme in buffer with 10 mM Mg²⁺ (lane Control B). B. Hydrolytic activity in % of hammerhead Spiegelzyme with the D-RNA target at different magnesium concentrations.