Figure 4. Hydrolysis of D-RNA and L-RNA substrates by hammerhead ribozymes and hammerhead Spiegelzymes.

The substrates (0.2 µM) were incubated with 2 µM of hammerhead ribozymes or hammerhead Spiegelzymes in 50 mM Tris-HCl, pH 7.5, buffer, at 37°C for 2 h. 10 mM MgCl₂ and hammerhead ribozymes or hammerhead Spiegelzymes were added as indicated. In the top panel, D-RNA substrates and in the bottom panel, L-RNA substrates were used. Hydrolysis products were separated on 20% polyacrylamide gel electrophoreses with 7 M urea. The ladder (right) was generated by alkaline hydrolysis.