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. 2014 Jan 29;9(1):e86673. doi: 10.1371/journal.pone.0086673

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© 2014 Wyszko et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. 5′-fluorescein-labelled Spiegelzyme (1 µg) was transfected into cells with Lipofectamine 2000. The microscope images were taken at 24, 72, 96, and 120 h after transfection. B. The RNA isolated from transfected cells (Trizol, Ambion) was analysed on 20% PAGE with 7 M urea. Lane 1 control hammerhead Spiegelzyme. Spiegelzyme after 120 h of incubation in COS-7 cells (lane 2).